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Differential White blood cell count 

White blood cells

Expected percentage (Adults)

Tally

Neutrophil

40-75 %

IIIII IIIII IIIII IIIII IIIII IIIII

Lymphocyte

21-40 %

IIIII IIIII IIIII I

Monocyte

2-10 %

IIII

Eosinophil

1-6 %

II

Basophil

0-1 %

0

Cheesbrough, M. (2005-2006)

Total number of White blood cell count is 53. Therefore, differential WBC count is as follows:

Neutrophils is equivalent to (30 / 53) X 100 = 56.6 %

Lymphocyte is equivalent to (16 / 53) X 100 = 30.2 %

Monocyte is equivalent to     (4 / 53) X 100 = 7.5 %

Eosinophil is equivalent to     (2 / 53) X 100 = 3.8 %

Basophil is equivalent to        (0 / 53) X 100 = 0 % 

Red Blood cell Count

The numbers of red cells counted in the haemocytometer using the solution with the dilution factor X100 are 7, 5 and 4.

Average number of red cells     = (12+ 5 + 7) / 3

                                               = 24 / 3

                                               = 8 red cells

Given that No. of cells per ml = Cell count X Dilution factor X 104, the number of cell count is                                                            = 8 X 100 X 104

                                                = 8 X 10red cells / ml of blood.

The numbers of red cells in the haemocytometer using the solution with the dilution factor X1000 are 3, 6 and 2

Average number of red cells       = (3 + 6 + 2) / 3

                                                 = 11 / 3

                                                 = 3.67 red cells

Given that No. of cells per ml = Cell count X Dilution factor X 104, the number of cell count is           

                                                 = 3.67 X 1000 X 104

                                                 = 3.67 x 107 red cells / ml of blood. 

Marching Blood Types for a blood Transfusion

Blood group matching is important to prevent any incompatibility between the donor’s and the recipient’s blood. Incompatibility arises when the donor’s blood has some incompatibility factors called the antigens (A, B, Rh factor) which trigger an immune response when they are foreign to a body (American Red Cross, 2012). These antigens result into the different blood groups existing in the body namely the ABO and the Rh blood group systems. The following table shows compatibility in the ABO blood group system for a safer transfusion:

Compatibility of the various ABO groups

 

 

 

DONOR

 

RECIPIENT

 

O

A

B

AB

O

A

 

 

B

 

 

AB

 

 

 

√- Means Compatibility

In the Rh blood group system, the Rh factor is the antigen and is represented by a positive (+) if present and a negative (-) if absent in the blood. Generally, an Rh (-) person receives blood from an Rh (-) donor while an Rh (+) positive person can receive blood from both an Rh (+/-) donor (American Red Cross, 2012). However, it is sometimes possible to mismatch the various blood groups thus producing severe consequences. One of the major consequences is an immune and clotting reaction which can lead to kidney failure, shock, circulatory collapse and even death. When an incompatible blood type is transfused, the immune system of the recipient will detect antigens, process it, and respond by removing the antigen from the recipient’s body (NCBI, 2009).

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